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| Petit, Fabienne; Clermont, Olivier; Delannoy, Sabine; Servais, Pierre; Gourmelon, Michele; Fach, Patrick; Oberle, Kenny; Fournier, Matthieu; Denamur, Erick; Berthe, Thierry. |
| The aim of this study was to investigate the diversity of the E. coli population, focusing on the occurrence of pathogenic E. coli, in surface water draining a rural catchment. Two sampling campaigns were carried out in similar hydrological conditions (wet period, low flow) along a river continuum, characterized by two opposite density gradients of animals (cattle and wild animals) and human populations. While the abundance of E. coli slightly increased along the river continuum, the abundance of both human and ruminant-associated Bacteroidales markers, as well as the number of E. coli multi-resistant to antibiotics, evidenced a fecal contamination originating from animals at upstream rural sites, and from humans at downstream urban sites. A strong spatial... |
| Tipo: Text |
Palavras-chave: Water; Sediment; Pathogenic E. coli; Virulence gene; Shiga toxin. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00378/48887/49328.pdf |
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| Shin, In-Sun; Nishikawa, Kiyotaka; Maruyama, Hiroki; Ishii, Satoshi. |
| A two-step binding assay for globotriaosylceramide (Gb3) content was developed by histidine-tagging strategy, which is a well-established method for the purification of recombinant proteins. The complete binding of the recombinant His-tagged Shiga toxin 1B subunit (1B-His) (1 microg/ml) to the standard Gb3 adsorbed on a multi-well H type plate was observed within 30 min at 37 degrees C; and its binding could be visualized by the following applications of HisProbe-HRP (8 microg/ml) and tetramethylbenzidine (TMB) peroxidase substrate. The 1B-His binding assay was linear over the range of 1 to 100 ng of Gb3 per well. The binding of 1B-His was specific to Gb3 separated from HeLa cells, and no major cross-reactivity of other glycolipids in Folch's lower... |
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Palavras-chave: Globotriaosylceramide; Shiga toxin; Binding assay method; His-tagged protein. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/833 |
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| Bergamini,Alzira Maria Morato; Simões,Marise; Irino,Kinue; Gomes,Tânia Aparecida Tardelli; Guth,Beatriz Ernestina Cabilio. |
| This study aims to assess the prevalence of Shiga toxin-producing Escherichia coli (STEC) in ground beef collected in two cities located in the State of São Paulo, Brazil. A total of 250 samples of raw ground beef were collected in local grocery stores during the period of March to December 2002 in the cities of Ribeirão Preto (114 samples) and Campinas (136 samples), São Paulo State, Brazil. The samples were processed according to standard methods. The resulting 591 E.coli colonies were screened for STEC by hybridization assays using the specific DNA probes, stx1,stx2 and eae. Further characterization of STEC isolates included the search for the ehxA sequence, detection of enterohemolysin and expression of Shiga toxin using the Vero cell assay. STEC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Escherichia coli; Shiga toxin; STEC; Serotypes; Ground beef. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300032 |
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